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        訂購信息
        上海拜力生物科技公司
        Tel:400-968-7988    021-33779008
        Monoclonal anti Calbindin D-28k 200ul
        品牌:Swant
        貨號:300
        規格:300
        貨期:swant

        瑞士swant?公司,瑞士一家生物科技公司,產品包括用于鈣結合蛋白(D-28k),鈣調蛋白,離子通道蛋白,神經遞質的抗體和抗原,用于神經元或神經膠質細胞及癌細胞的染色產品和用于免疫組合的單抗和多抗。多年來專注于鈣結合蛋白產品的研究,其產品雖種類雖然不多,但是卻都經典熱銷,并且經常出現在高分的文章中。為國內外眾多相關領域科學家所熱衷。

        商品詳情 參考文獻 相關資料

        產品介紹

        品名

        Monoclonal anti Calbindin D-28k 

        貨號

        300

        規格

        200ul / 500ul  / 1ml

         克隆性

        monoclonal

        共軛

        nonconjugated

        反應性

        thirteen-lined ground squirrel, Spanish newt, slender lungfish, reedfish , Shaw's jird, gray bichir , African green monkey, human, mouse, rat, chicken, pigs , Xenopus laevis, rhesus macaque

        應用

        western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

        宿主動物

        Mouse monoclonal, IgG1

        抗體形式

        Conc. supernat. lyophil.

        品形式

        Lyophilized concentrated supernatant (no preservatives). 

        復溶

        with 200 μl / 500 μl or 1 ml bidistilled water

        產品描述

        Monoclonal anti-calbindin D-28k is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with calbindin D-28k purified from chicken gut (1). The antibody was evaluated for specificity and potency: a) by indirect immunofluorescent or immunoperoxidase labelling as well as Biotin-Avidin labeling of cryostate or vibratome-sections of 4 % paraformaldehyde fixed tissue; b) by immunoenzymatic labelling of immunoblots; c) by radioimmunoassay. 

        The product is a monoclonal antibody (McAB) against calbindin D-28k, a calcium-binding protein of the EF-hand family related to calmodulin and troponin-C

        稀釋

        Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method.

        Immunoblots: 1:100 - 1:1'000 with an indirect peroxidase method. 

        We recommend that the optimal dilutions be determined by titration experiments.

        儲存

        For long storage, keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing. 

        參考文獻

        1. Celio M.R. et al., Cell Calcium 11:599-602, 1990 

        2. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press 

        3. Garcia-Segura L.M. et al. (1984) Brain Rews. 296: 75-86. 

        4. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493

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